Microfluidic approaches to synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy of living biosystems

Author(s): Kevin Loutherback, Giovanni Birarda, Liang Chen and Hoi-Ying N. HolmanPages 273-282 (10) Abstract: A long-standing desire in biological and biomedical sciences is to be able to probe cellular chemistry as biological processes are happening inside living cells. Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy is a label-free...

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Kinetics for Zinc Ion Induced Sepia Pharaonis Arginine Kinase Inactivation and Aggregation

Author(s): Yue-Xiu Si, Jinhyuk Lee, Juan-Ge Cheng, Shang-Jun Yin, Yong-Doo Park, Guo-Ying Qian and Xia-Min JiangPages 508-517 (10) Abstract: Arginine kinase is an essential enzyme which is closely related to energy metabolism in marine invertebrates. Arginine kinase provides a significant role in quick response to environmental change and stress. In this study, we simulated...

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The Influence of Ionizing Radiation on Exosome Composition, Secretion and Intercellular Communication

Author(s): Karol Jelonek, Piotr Widlak and Monika PietrowskaPages 656-663 (8) Abstract: A large variety of vesicles is actively secreted into the extracellular space by most type of cells. The smallest nanoparticles (30-120 nm), called exosomes, are known to transport their cargo (nucleic acids, proteins and lipids) between diverse locations in the body. Specific content...

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Cell-Free Technologies for Proteomics and Protein Engineering

Author(s): Hiroyuki Ohashi and Etsuko Miyamoto-SatoPages 819-827 (9) Abstract: Cell-free translation systems facilitate rapid production of specific proteins and are particularly suited as high-throughput methods for whole-genome protein synthesis. Moreover, these systems do not rely on living cells, thereby allowing the synthesis of unstable or cytotoxic proteins in...

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