Most Cited Article: Novel Antimicrobial Peptides Isolated from Skin Secretions of the Mexican Frog Hyla eximia

Novel Antimicrobial Peptides Isolated from Skin Secretions of the Mexican Frog Hyla eximia

Author(s):

Oscar Villa-Hernandez, Lorena Hernandez-Orihuela, Maria del Carmen Rodriguez, Fernando Zamudio-Zuniga, Ruben Castro-Franco, Victoria Pando and Cesar V.F. Batista   Pages 1371 - 1378 ( 8 )

Abstract:

In this work, we describe the original characterization of peptides and proteins present in the skin secretions of the Mexican amphibian Hyla eximia. To this purpose, a novel water/dark extraction method, as well as the classic electrical stimulation procedure, was applied in order to extract the skin secretion. Two novel antimicrobial peptides He-1 and He-2 were sequenced. In addition, a molecular mass fingerprint revealed more than one hundred different molecules. Eight peptides in homogeneous form were assayed against five species of bacteria. Thereafter, the peptide He-2 demonstrated high antiparasitic activity against ookinete forms of malaria parasites at low concentration.

Keywords:

Hyla eximia, antimicrobial peptides, mass spectrometry

Affiliation:

Unidad de Proteomica, Instituto de Biotecnologia-UNAM, Av. Universidad, 2001, Col. Chamilpa, CP 62210, Cuernavaca, Mor, Mexico.

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